Development, Validation and simultaneous estimation of Sulfamethoxazole and Trimethoprim by RP-HPLC Method
Abstract
The straightforward, precise, accurate approach has been established for such simultaneous determination of a Sulfamethoxazole as well as Trimethoprim through oral dosage form. Chromatogram has been operate via inertsil ODS C185µm (4.6 x 250mm). Mobile phase comprising phosphate - buffered as well as acetonitrile with in proportion like 30:70 has been fired up thru section at such a rate of flow like 1ml/min. Buffer in use at pH 4.6, temperature has been retained there as ambient. Optimization frequency range such as Sulfamethoxazole as well as Trimethoprim has been 249nm. Time of retention like Sulfamethoxazole as well as trimethoprim have been did find of being 2.327min as well as 4.342min. A % purification like Sulfamethoxazole as well as Trimethoprim has been did find of being 100.6% as well as 101.3% including both. a stability studies parametric such as Sulfamethoxazole as well as Trimethoprim like theoretical plates as well as tailing element have been did find of being 1.3, 5117.5.as well as 1.4, 3877.3 a resolution has been did find of being 4.1.a linear relationship research such as Sulfamethoxazole as well as Trimethoprim has been present in range of concentrations like 20 ppm-100 ppm but also 10 ppm-50 ppm as well as coefficient of correlation (r2) has been did find of being 0.9999 as well as 0.9998, % average retrieval has been did find of about 99.96% as well as 99.75%, % RSD such as reproducibility was 0.31 as well as 0.38, % RSD such as medium precision has been 0.12 but also 0.15 including both. A precision research was accurate, robust as well as reproducible. LOD valuation has been 0.48 as well as 0.55, but also LOQ valuation has been 0.95 but also 1.10 including both.
References
2. Skoog DA, Holler FJ, and Crouch SR. Textbook of Instrumental Analysis. Brook/Cole. Cengage Learning India Private Limited; 2007.p.900-906.
3. Gurumurthy.T, Monika.M, and Ashwini.V. Development and validation RP-HPLC method for simultaneous estimation of Sulfamethoxazole and Trimethoprim. Indian Journal of Research in Pharmacy and Biotechnology. 2017;5(3):235-238.
4. Goulas V, Anisimova Andreou T, Angastinioti Moditi C and Tzamaloukas O. A Rapid HPLC Method for the Determination of Sulphonamides and Trimethoprim in Feed Premixes. Journal of Animal and Feed Sciences. 2014;23:185-189.
5. Jyothi M, Varaprasad A, Vandana B, Nikitha G, Sandhya S, and Sukanya U. RP-HPLC Method Designed for Determining Charantin in Its Capsule Dosage Form. Future Journal of Pharmaceuticals and Health Sciences. 2021;1(3):118–122.
6. Jing-Chun Wang, Qi Zhang, and De-Fu Cai. Stability-Indicating Validated HPLC Method for Analysis of Berberine Hydrochloride and Trimethoprim in Pharmaceutical Dosage Form. Journal of Chemistry. 2013;9.
7. Mashhour M Ghanem and Saleh A Abu-Lafi. Development and validation of a stability-indicating HPLC method for the simultaneous determination of sulfadiazine sodium and trimethoprim in injectable solution formulation. Scientia Pharmaceutica. 2013; 81(1):167-182.
8. Sayyed Nazifa S, Patel Seema A, Manjra Mehfuza U, Lajporiya Mobina I, Aejaz Ahmed A, Khan G J, Quazi Majaz and Patel M Siddik. A Simple UV-Vis Spectrophotometric Assay study on different brands of Mefenamic Acid, Paracetamol and Furosemide. Future Journal of Pharmaceuticals and Health Sciences; 2(2):57-62.
9. Patel RB and Welling PG. Clinical pharmacokinetics of co-trimoxazole (trimethoprim-sulphamethoxazole). Clinical Pharmacokinetics. 1980; 5(5):405-423.
10. Eliopolos GM and Wennersten CB. In vitro activity of trimethoprim alone compared with trimethoprim-Sulfamethoxazole and other anti-microbials against bacterial species associated with upper respiratory tract infection. Diagnostic Microbiology and Infectious Disease. 1997;29(1):33-38.
11. Banothu Srikanth, Greg Maryann Nzubechuwku, Bello Munirat Omowumi, Jacintah David Kolo, & Zoya Fatima. RP-HPLC Method Development and Validation for the Simultaneous Estimation of Ivermectin and Albendazole in its Pure and Combine Dosage Form. Future Journal of Pharmaceuticals and Health Sciences. 2022;2(3):170–184.
12. Richards RM and Xing JZ. Mechanism of sulphadiazine enhancement of trimethoprim activity against sulphadiazine resistant Enterococcus faecalis. The Journal of Antimicrobial Chemotherapy. 1995; 36(4):607-618.
13. Hale E, Habtegarbr E, and Mcqueen R. Co-trimoxazole for the treatment of listeriosis and its successful use in a patient with AIDS. The Journal of Infection. 1994;28(1):110-113.
14. Behzadian Nejad, Rezaee AG and Kebriae ezadeh A. High-performance liquid chromatographic determination of trimethoprim in mouse liver. Pharmacy and Pharmacology Communications. 1998;4:439-441.
15. Balakrishnan M, Melapudi Krishna Reddy Manisha, & Monika P S. HPTLC Method in Determination of Guggulosterone Z from Leaf Extract of Tribulus terrestris Linn. Future Journal of Pharmaceuticals and Health Sciences.2022;2(3):125-129.
16. Bowen AC, Lilliebridge RA, Tong SY, Baird RW, Ward P, McDonald MI, Currie BJ, Carapetis JR. Is Streptococcus pyogenes resistant or susceptible to trimethoprim-Sulfamethoxazole. Journal of Clinical Microbiology. 2012;50(12):4067-72.
17. Sudheer Kumar H M and Kothapalli B C. Stability Indicating Analytical Technique Development and Validation for the Determination of Fexinidazole in Bulk and Dosage Form Utilizing RP-HPLC. Future Journal of Pharmaceuticals and Health Sciences. 2022; 2(4):293–300.
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